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dc.creatorCañada Vicinay, Francisco Javier-
dc.creatorCorrales, Guillermo-
dc.creatorFernández-Mayoralas, Alfonso-
dc.creatorMartín-Lomas, Manuel-
dc.creatorAragón, Juan J.-
dc.date2008-01-16T17:33:39Z-
dc.date2008-01-16T17:33:39Z-
dc.date2006-03-29-
dc.date.accessioned2017-01-31T00:59:43Z-
dc.date.available2017-01-31T00:59:43Z-
dc.identifierEuropean Patent Code: EP 1408118 B1-
dc.identifierhttp://hdl.handle.net/10261/2698-
dc.identifier.urihttp://dspace.mediu.edu.my:8181/xmlui/handle/10261/2698-
dc.descriptionFiling Date: 2002-06-14.-- Priority Data: ES 200101419 (2001-06-18).-- International Publication Number: WO_2002103038 (20021227).-
dc.descriptionAn enzymatic process to obtain 4-O-β-D-galactopyranosyl-D-xylose useful in compositions or solutions in the in vivo evaluation of intestinal lactose activity in humans, that comprises the steps of preparing a reaction mixture of D-xylose, a β-D-galactopyranoside and a reaction medium that comprises water buffered to a pH between 5.0 and 9.0; adding 10 to 1,000 units of β-D-galactosidase per gram of β-D-galactopyranoside; subjecting the reaction mixture to a reaction or a temperature between a temperature higher than the freezing point of the reaction mixture and 45ºC, for 2 to 48 hours; for the reaction by deactivation of the β-D-galactosidase; and to isolate and crystallize the fractions that contain 4-O-β-D-galactopyranosyl-D-xylose from a crystallization mixture selected between mixtures of acetone/methanol in a ratio between 5/1 to 20/1 and mixtures of acetone/water in a ratio between 5/1 to 20/1.-
dc.format185663 bytes-
dc.formatapplication/pdf-
dc.languageeng-
dc.rightsopenAccess-
dc.titleEnzymatic method of producing 4-O- β-D-galactopyranosyl-D-xylose, 4-O- β-D-galactopyranosyl-D-xylose obtained using said method, compositions containing said and the use thereof in evaluating intestinal lactase-
dc.typePatente-
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