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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2841| Title: | Putative Porin of Bradyrhizobium sp. (Lupinus) Bacteroids Induced by Glyphosate |
| Publisher: | American Society for Microbiology |
| Description: | Application of glyphosate (N-[phosphonomethyl] glycine) to Bradyrhizobium sp. (Lupinus)-nodulated lupin plants caused modifications in the protein pattern of bacteroids. The most significant change was the presence of a 44-kDa polypeptide in bacteroids from plants treated with the higher doses of glyphosate employed (5 and 10 mM). The polypeptide has been characterized by the amino acid sequencing of its N terminus and the isolation and nucleic acid sequencing of its encoding gene. It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a transmembrane beta-barrel. The protein has been designated BLpp, for Bradyrhizobium sp. (Lupinus) putative porin, and would be the first porin described in Bradyrhizobium sp. (Lupinus). In addition, a putative conserved domain of porins has been identified which consists of 87 amino acids, located in the BLpp sequence 30 amino acids downstream of the N-terminal region. In bacteroids, mRNA of the BLpp gene shows a basal constitutive expression that increases under glyphosate treatment, and the expression of the gene is seemingly regulated at the transcriptional level. By contrast, in free-living bacteria glyphosate treatment leads to an inhibition of BLpp mRNA accumulation, indicating a different effect of glyphosate on BLpp gene expression in bacteroids and free-living bacteria. The possible role of BLpp in a metabolite interchange between Bradyrhizobium and lupin is discussed. This work was supported by grants 142/CH-39 and 168/CH-47 from Junta de Comunidades de Castilla-La Mancha (C-LM) and BIO2004-04968-C02 from CICYT. N.D.M. was supported by a C-LM predoctoral fellowship. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2841 |
| Other Identifiers: | Applied and Environmental Microbiology: 73(16): 5075–5082 (2007) 0099-2240 http://hdl.handle.net/10261/2841 10.1128/AEM.00392-07 1098-5336 |
| Appears in Collections: | Digital Csic |
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