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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2841Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | María, Nuria de | - |
| dc.creator | Guevara, Angeles | - |
| dc.creator | Serra, María Teresa | - |
| dc.creator | García-Luque, Isabel | - |
| dc.creator | González-Sama, Alfonso | - |
| dc.creator | García de Lacoba, Mario | - |
| dc.creator | Felipe, Mª Rosario de | - |
| dc.creator | Fernández-Pascual, Mercedes | - |
| dc.date | 2008-02-04T18:48:48Z | - |
| dc.date | 2008-02-04T18:48:48Z | - |
| dc.date | 2007-06-08 | - |
| dc.date.accessioned | 2017-01-31T00:59:56Z | - |
| dc.date.available | 2017-01-31T00:59:56Z | - |
| dc.identifier | Applied and Environmental Microbiology: 73(16): 5075–5082 (2007) | - |
| dc.identifier | 0099-2240 | - |
| dc.identifier | http://hdl.handle.net/10261/2841 | - |
| dc.identifier | 10.1128/AEM.00392-07 | - |
| dc.identifier | 1098-5336 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2841 | - |
| dc.description | Application of glyphosate (N-[phosphonomethyl] glycine) to Bradyrhizobium sp. (Lupinus)-nodulated lupin plants caused modifications in the protein pattern of bacteroids. The most significant change was the presence of a 44-kDa polypeptide in bacteroids from plants treated with the higher doses of glyphosate employed (5 and 10 mM). The polypeptide has been characterized by the amino acid sequencing of its N terminus and the isolation and nucleic acid sequencing of its encoding gene. It is putatively encoded by a single gene, and the protein has been identified as a putative porin. Protein modeling revealed the existence of several domains sharing similarity to different porins, such as a transmembrane beta-barrel. The protein has been designated BLpp, for Bradyrhizobium sp. (Lupinus) putative porin, and would be the first porin described in Bradyrhizobium sp. (Lupinus). In addition, a putative conserved domain of porins has been identified which consists of 87 amino acids, located in the BLpp sequence 30 amino acids downstream of the N-terminal region. In bacteroids, mRNA of the BLpp gene shows a basal constitutive expression that increases under glyphosate treatment, and the expression of the gene is seemingly regulated at the transcriptional level. By contrast, in free-living bacteria glyphosate treatment leads to an inhibition of BLpp mRNA accumulation, indicating a different effect of glyphosate on BLpp gene expression in bacteroids and free-living bacteria. The possible role of BLpp in a metabolite interchange between Bradyrhizobium and lupin is discussed. | - |
| dc.description | This work was supported by grants 142/CH-39 and 168/CH-47 from Junta de Comunidades de Castilla-La Mancha (C-LM) and BIO2004-04968-C02 from CICYT. N.D.M. was supported by a C-LM predoctoral fellowship. | - |
| dc.description | Peer reviewed | - |
| dc.format | 635775 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society for Microbiology | - |
| dc.relation | http://dx.doi.org/10.1128/AEM.00392-07 | - |
| dc.rights | openAccess | - |
| dc.title | Putative Porin of Bradyrhizobium sp. (Lupinus) Bacteroids Induced by Glyphosate | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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