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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2889Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Rubio, Daniel | - |
| dc.creator | García, Silvia | - |
| dc.creator | Paz, María F. | - |
| dc.creator | Cueva, Teresa de la | - |
| dc.creator | López-Fernández, Luis A. | - |
| dc.creator | Lloyd, Alison C. | - |
| dc.creator | García-Castro, Javier | - |
| dc.creator | Bernad, Antonio | - |
| dc.date | 2008-02-07T00:09:08Z | - |
| dc.date | 2008-02-07T00:09:08Z | - |
| dc.date | 2008-01-02 | - |
| dc.date.accessioned | 2017-01-31T01:00:00Z | - |
| dc.date.available | 2017-01-31T01:00:00Z | - |
| dc.identifier | PLoS ONE. 2008; 3(1): e1398. | - |
| dc.identifier | 1932-6203 | - |
| dc.identifier | http://hdl.handle.net/10261/2889 | - |
| dc.identifier | 10.1371/journal.pone.0001398 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2889 | - |
| dc.description | Background. We previously reported the in vitro spontaneous transformation of human mesenchymal stem cells (MSC) generating a population with tumorigenic potential, that we termed transformed mesenchymal cells (TMC). | - |
| dc.description | Methodology/Principal Findings. Here we have characterized the molecular changes associated with TMC generation. Using microarrays techniques we identified a set of altered pathways and a greater number of downregulated than upregulated genes during MSC transformation, in part due to the expression of many untranslated RNAs in MSC. Microarray results were validated by qRT-PCR and protein detection. | - |
| dc.description | Conclusions/Significance. In our model, the transformation process takes place through two sequential steps; first MSC bypass senescence by upregulating c-myc and repressing p16 levels. The cells then bypass cell crisis with acquisition of telomerase activity, Ink4a/Arf locus deletion and Rb hyperphosphorylation. Other transformation-associated changes include modulation of mitochondrial metabolism, DNA damage-repair proteins and cell cycle regulators. In this work we have characterized the molecular mechanisms implicated in TMC generation and we propose a two-stage model by which a human MSC becomes a tumor cell. | - |
| dc.description | DR and SG received predoctoral fellowships from the Spanish Ministry of Education and Science, JG-C and L L-F received postdoctoral fellowships from the Ministry of Science and Technology and the Ministerio de Sanidad y Consumo (FIS; CP03/0031 and CP06/0267). This work was partially supported by Spanish Ministry of Science and Technology (CICYT) grants SAF2001-2262, SAF2005-0864 and GEN2001-4856-C13-02 to AB. The Department of Immunology and Oncology was founded and is supported by the Spanish National Research Council (CSIC) and by Pfizer. | - |
| dc.description | Peer reviewed | - |
| dc.format | 1055768 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Public Library of Science | - |
| dc.relation | Publisher’s version | - |
| dc.rights | openAccess | - |
| dc.title | Molecular Characterization of Spontaneous Mesenchymal Stem Cell Transformation | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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