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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Roncel Gil, Mercedes | - |
| dc.creator | Yruela Guerrero, Inmaculada | - |
| dc.creator | Kirilovsky, D. | - |
| dc.creator | Guerrero, Fernando | - |
| dc.creator | Alfonso Lozano, Miguel | - |
| dc.creator | Picorel Castaño, Rafael | - |
| dc.creator | Ortega, José M. | - |
| dc.date | 2008-02-11T13:24:52Z | - |
| dc.date | 2008-02-11T13:24:52Z | - |
| dc.date | 2007-06 | - |
| dc.date.accessioned | 2017-01-31T01:00:06Z | - |
| dc.date.available | 2017-01-31T01:00:06Z | - |
| dc.identifier | Biochimica et Biophysica Acta (BBA) - Bioenergetics 1767 (2007) 694-702. | - |
| dc.identifier | 0005-2728 | - |
| dc.identifier | http://hdl.handle.net/10261/2917 | - |
| dc.identifier | 10.1016/j.bbabio.2007.02.017 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/2917 | - |
| dc.description | The definitive version is available at: http://www.sciencedirect.com/science//journal/00052728 | - |
| dc.description | Anomalies in photosynthetic activity of the soybean cell line STR7, carrying a single mutation (S268P) in the chloroplastic gene psbA that codes for the D1 protein of the photosystem II, have been examined using different spectroscopic techniques. Thermoluminescence emission experiments have shown important differences between STR7 mutant and wild type cells. The afterglow band induced by both white light flashes and far-red continuous illumination was downshifted by about 4 °C and the Q band was upshifted by 5 °C. High temperature thermoluminescence measurements suggested a higher level of lipid peroxidation in mutant thylakoid membranes. In addition, the reduction rate of P700+ was significantly accelerated in STR7 suggesting that the mutation led to an activation of the photosystem I cyclic electron flow. Modulated fluorescence measurements performed at room temperature as well as fluorescence emission spectra at 77 K revealed that the STR7 mutant is defective in state transitions. Here, we discuss the hypothesis that activation of the cyclic electron flow in STR7 cells may be a mechanism to compensate the reduced activity of photosystem II caused by the mutation. We also propose that the impaired state transitions in the STR7 cells may be due to alterations in thylakoid membrane properties induced by a low content of unsaturated lipids. | - |
| dc.description | This work was supported by grants from the Ministry of Education and Culture of Spain (BFU-BMC2004-04914-C02-01, BMC2002-00031 and BFU-BMC2005-07422-C02-01) and Andalusia Government (PAI CVI-261). | - |
| dc.description | Peer reviewed | - |
| dc.format | 321411 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.rights | openAccess | - |
| dc.subject | Cyclic and linear electron flow | - |
| dc.subject | Photosystem I | - |
| dc.subject | Photosystem II | - |
| dc.subject | Thermoluminiscence | - |
| dc.subject | State transitions | - |
| dc.subject | D1 mutant | - |
| dc.title | Changes in photosynthetic electron transfer and state transitions in an herbicide-resistant D1 mutant from soybean cell cultures | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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