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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3242Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Lacalle, Rosa Ana | - |
| dc.creator | Peregil, Rosa M. | - |
| dc.creator | Albar, Juan Pablo | - |
| dc.creator | Merino Plaza, Ernesto | - |
| dc.creator | Martínez-Alonso, Carlos | - |
| dc.creator | Mérida, Isabel | - |
| dc.creator | Mañes, Santos | - |
| dc.date | 2008-03-18T12:13:38Z | - |
| dc.date | 2008-03-18T12:13:38Z | - |
| dc.date | 2007-12 | - |
| dc.date.accessioned | 2017-01-31T01:00:46Z | - |
| dc.date.available | 2017-01-31T01:00:46Z | - |
| dc.identifier | The Journal of Cell Biology, Vol. 179, No. 7, pp. 1539-1553 | - |
| dc.identifier | 0021-9525 | - |
| dc.identifier | http://hdl.handle.net/10261/3242 | - |
| dc.identifier | 10.1083/jcb.200705044 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3242 | - |
| dc.description | Copyright by The Rockefeller University Press | - |
| dc.description | Directional cell movement in response to external chemical gradients requires establishment of front–rear asymmetry, which distinguishes an up-gradient protrusive leading edge, where Rac-induced F-actin polymerization takes place, and a down-gradient retractile tail (uropod in leukocytes), where RhoA-mediated actomyosin contraction occurs. The signals that govern this spatial and functional asymmetry are not entirely understood. We show that the human type I phosphatidylinositol 4-phosphate 5-kinase isoform β (PIPKIβ) has a role in organizing signaling at the cell rear. We found that PIPKIβ polarized at the uropod of neutrophil-differentiated HL60 cells. PIPKIβ localization was independent of its lipid kinase activity, but required the 83 C-terminal amino acids, which are not homologous to other PIPKI isoforms. The PIPKIβ C terminus interacted with EBP50 (4.1-ezrin-radixin-moesin (ERM)-binding phosphoprotein 50), which enabled further interactions with ERM proteins and the Rho-GDP dissociation inhibitor (RhoGDI). Knockdown of PIPKIβ with siRNA inhibited cell polarization and impaired cell directionality during dHL60 chemotaxis, suggesting a role for PIPKIβ in these processes. | - |
| dc.description | Peer reviewed | - |
| dc.format | 404731 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Rockefeller University Press | - |
| dc.rights | openAccess | - |
| dc.title | Type I phosphatidylinositol 4-phosphate 5-kinase controls neutrophil polarity and directional movement | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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