1. DSpace at MEDIU
  2. Harvested articles مقالات مستوردة من مؤسسات وجامعات عالمية
  3. Digital Csic
Please use this identifier to cite or link to this item: http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3316
Full metadata record
DC FieldValueLanguage
dc.creatorGómez-Moutón, Concepción-
dc.creatorLacalle, Rosa Ana-
dc.creatorMira, Emilia-
dc.creatorJiménez Baranda, Sonia-
dc.creatorBarber, Domingo F.-
dc.creatorCarrera, Ana C.-
dc.creatorMartínez-Alonso, Carlos-
dc.creatorMañes, Santos-
dc.date2008-03-26T12:56:06Z-
dc.date2008-03-26T12:56:06Z-
dc.date2004-03-
dc.date.accessioned2017-01-31T01:00:57Z-
dc.date.available2017-01-31T01:00:57Z-
dc.identifierThe Journal of Cell Biology, Volume 164, Number 5, March 1, 2004, pp. 759–768-
dc.identifier0021-9525-
dc.identifierhttp://hdl.handle.net/10261/3316-
dc.identifier10.1083/jcb.200309101-
dc.identifier.urihttp://dspace.mediu.edu.my:8181/xmlui/handle/10261/3316-
dc.descriptionCopyright © by The Rockefeller University Press-
dc.descriptionSpatially restricted activation of signaling molecules governs critical aspects of cell migration; the mechanism by which this is achieved nonetheless remains unknown. Using time-lapse confocal microscopy, we analyzed dynamic redistribution of lipid rafts in chemoattractant-stimulated leukocytes expressing glycosyl phosphatidylinositol– anchored green fluorescent protein (GFP-GPI). Chemoattractants induced persistent GFP-GPI redistribution to the leading edge raft (L raft) and uropod rafts of Jurkat, HL60, and dimethyl sulfoxide–differentiated HL60 cells in a pertussis toxin–sensitive, actin-dependent manner. A trans-S membrane, nonraft GFP protein was distributed homogeneously in moving cells. A GFP-CCR5 chimera, which partitions in L rafts, accumulated at the leading edge, and CCR5 redistribution coincided with recruitment and activation of phosphatidylinositol-3 kinase in L rafts in polarized, moving cells. Membrane cholesterol depletion impeded raft redistribution and asymmetric recruitment of PI3K to the cell side facing the chemoattractant source. This is the first direct evidence that lipid rafts order spatial signaling in moving mammalian cells, by concentrating the gradient sensing machinery at the leading edge.-
dc.descriptionPeer reviewed-
dc.format4235742 bytes-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherRockefeller University Press-
dc.rightsopenAccess-
dc.titleDynamic redistribution of raft domains as an organizing platform for signaling during cell chemotaxis-
dc.typeArtículo-
Appears in Collections:Digital Csic

Files in This Item:
There are no files associated with this item.
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.