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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3341| Title: | Improved resistance to controlled deterioration in transgenic seeds. |
| Authors: | Ministerio de Educación y Ciencia (España) Junta de Andalucía |
| Keywords: | Heat-Shok Proteins Stress Transcription Factors Oryza-Sativa L. Heat-Shock Proteins Stress Transcription Desication Tolerance Arabidopsis-Thaliana Abscisic acid (ABA) Longevity Expression Thermotolerance Embryogenesis |
| Publisher: | American Society of Plant Biologists |
| Description: | This article has been evaluated in Faculty of 1000 Biology. ISSN 1740-4118 We show that seed-specific overexpression of the sunflower (Helianthus annuus) HaHSFA9 heat stress transcription factor (HSF) in tobacco (Nicotiana tabacum) enhances the accumulation of heat-shock proteins (HSPs). Among these proteins were HSP101 and a subset of the small HSPs (sHSPs), including proteins that accumulate only during embryogenesis in the absence of thermal stress. Levels of Late Embryogenesis Abundant proteins or seed oligosaccharides, however, were not affected. In the transgenic seeds, a high basal thermotolerance persisted during the early hours of imbibition. Transgenic seeds also showed significantly improved resistance to controlled deterioration in a stable and transgene-dependent manner. Furthermore, overexpression of HaHSFA9 did not have detrimental effects on plant growth or development, including seed morphology and total seed yield. Our results agree with previous work tentatively associating HSP gene expression with phenotypes important for seed longevity. These findings might have implications for improving seed longevity in economically important crops. This work was supported by the Spanish Ministry of Education and Science (grants no. BIO02-1463 and BIO05-0949). We also received partial support from the Andalusian Regional Government (“Junta de Andalucía”, grant CVI148). Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3341 |
| Other Identifiers: | Plant Physiology 142:1102-1112 (2006) 0032-0889 http://hdl.handle.net/10261/3341 10.1104/pp.106.087817 |
| Appears in Collections: | Digital Csic |
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