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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3394| Title: | Genomic analysis reveals the major driving forces of bacterial life in the rhizosphere |
| Publisher: | BioMed Central |
| Description: | The electronic version of this article is the complete one and can be
found online at http://genomebiology.com/2007/8/9/R179 [Background] Mutualistic interactions less well known than those between rhizobia and legumes are commonly found between plants and bacteria, frequently pseudomonads, which colonize roots and adjacent soil areas (the rhizosphere). [Results] A global analysis of Pseudomonas putida genes expressed during their interaction with maize roots revealed how a bacterial population adjusts its genetic program to this lifestyle. Differentially expressed genes were identified by comparing rhizosphere-colonizing populations with three distinct controls covering a variety of nutrients, growth phases and life styles (planktonic and sessile). Ninety rhizosphere up-regulated (rup) genes, which were induced relative to all three controls, were identified, whereas there was no repressed gene in common between the experiments. Genes involved in amino acid uptake and metabolism of aromatic compounds were preferentially expressed in the rhizosphere, which reflects the availability of particular nutrients in root exudates. The induction of efflux pumps and enzymes for glutathione metabolism indicates that adaptation to adverse conditions and stress (oxidative) response are crucial for bacterial life in this environment. The finding of a GGDEF/EAL domain response regulator among the induced genes suggests a role for the turnover of the secondary messenger c-diGMP in root colonization. Several mutants in rup genes showed reduced fitness in competitive root colonization. [Conclusion] Our results show the importance of two selective forces of different nature to colonize the rhizosphere: stress adaptation and availability of particular nutrients. We also identify new traits conferring bacterial survival in this niche and open a way to the characterization of specific signalling and regulatory processes governing the plant-Pseudomonas association. This work was supported by grants GEN2001-4698-CO5-03, BIO2003-0515, and BFU2006-09078 from the Plan Nacional de I+D+I. MAM is the recipient of a fellowship from the Ministerio de Educación y Ciencia. Research contract and funding from the Junta de Andalucía to MIR-G are also acknowledged. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3394 |
| Other Identifiers: | Genome Biology 2007, 8:R179 1465-6914 http://hdl.handle.net/10261/3394 10.1186/gb-2007-8-9-r179 |
| Appears in Collections: | Digital Csic |
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