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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Campo, Rosa Del | - |
| dc.creator | Tenorio, Carmen | - |
| dc.creator | Jiménez Díaz, Rufino | - |
| dc.creator | Rubio, Carmen | - |
| dc.creator | Gómez-Lus, Rafael | - |
| dc.creator | Baquero, Fernando | - |
| dc.creator | Torres, Carmen | - |
| dc.date | 2008-04-11T07:19:18Z | - |
| dc.date | 2008-04-11T07:19:18Z | - |
| dc.date | 2001-03 | - |
| dc.date.accessioned | 2017-01-31T01:01:59Z | - |
| dc.date.available | 2017-01-31T01:01:59Z | - |
| dc.identifier | Antimicrobial Agents and Chemotherapy 45(3): 905–912 (2001) | - |
| dc.identifier | 1098-6596 | - |
| dc.identifier | http://hdl.handle.net/10261/3533 | - |
| dc.identifier | 10.1128/AAC.45.3.905-912.2001 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3533 | - |
| dc.description | Bacteriocin production was determined for 218 Enterococcus isolates (Enterococcus faecalis [93] and E. faecium [125]) obtained from different origins (human clinical samples [87], human fecal samples [78], sewage [28], and chicken samples [25]) and showing different vancomycin susceptibility patterns (vancomycin resistant, all of them vanA positive [56], and vancomycin susceptible [162]). All enterococcal isolates were randomly selected except for the vancomycin-resistant ones. A total of 33 isolates of eight different bacterial genera were used as indicators for bacteriocin production. Forty-seven percent of the analyzed enterococcal isolates were bacteriocin producers (80.6% of E. faecalis and 21.6% of E. faecium isolates). The percentage of bacteriocin producers was higher among human clinical isolates (63.2%, 81.8% of vancomycin-resistant isolates and 60.5% of vancomycin-susceptible ones) than among isolates from the other origins (28 to 39.3%). Only one out of the 15 vancomycin-resistant isolates from human fecal samples was a bacteriocin producer, while 44.4% of fecal vancomycin-susceptible isolates were. The bacteriocin produced by the vanA-containing E. faecium strain RC714, named bacteriocin RC714, was further characterized. This bacteriocin activity was cotransferred together with the vanA genetic determinant to E. faecalis strain JH2-2. Bacteriocin RC714 was purified to homogeneity and its primary structure was determined by amino acid sequencing, showing an identity of 88% and a similarity of 92% with the previously described bacteriocin 31 from E. faecalis YI717. The presence of five different amino acids in bacteriocin RC714 suggest that this could be a new bacteriocin. The results obtained suggest that the epidemiology of vancomycin resistance may be influenced by different factors, including bacteriocin production. | - |
| dc.description | R. D. C. was supported by a grant from the Diputación General de Aragón of Spain (project P49/97) and from the Sociedad Española de Quimioterapia. This work has been supported in part by a grant from the Fondo de Investigaciones Sanitarias (00/0545) of Spain. | - |
| dc.description | Peer reviewed | - |
| dc.format | 320677 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society for Microbiology | - |
| dc.rights | closedAccess | - |
| dc.title | Bacteriocin Production in Vancomycin-Resistant and Vancomycin-Susceptible Enterococcus Isolates of Different Origins | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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