1. DSpace at MEDIU
  2. Harvested articles مقالات مستوردة من مؤسسات وجامعات عالمية
  3. Digital Csic
Please use this identifier to cite or link to this item: http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3685
Full metadata record
DC FieldValueLanguage
dc.creatorToribio, María Luisa-
dc.creatorHera, Antonio de la-
dc.creatorBorst, Jannie-
dc.creatorBorst, Jannie-
dc.creatorMarcos, Miguel A. R.-
dc.creatorMárquez, Carlos-
dc.creatorAlonso, José M.-
dc.creatorBárcena, Alicia-
dc.creatorMartínez-Alonso, Carlos-
dc.date2008-04-23T07:52:35Z-
dc.date2008-04-23T07:52:35Z-
dc.date1988-12-
dc.date.accessioned2017-01-31T01:03:03Z-
dc.date.available2017-01-31T01:03:03Z-
dc.identifierThe Journal of Experimental Medicine, Volume 168, December 1988, pp. 2231-2249-
dc.identifier0022-1007-
dc.identifierhttp://hdl.handle.net/10261/3685-
dc.identifier.urihttp://dspace.mediu.edu.my:8181/xmlui/handle/10261/3685-
dc.descriptionEl copyright pertenece a The Rockefeller University Press-
dc.descriptionT cell precursors arising from hematopoietic stem cells colonize the thymus during ontogeny, where they undergo a complex maturational process involving genotypic and phenotypic changes in the expression of distinct surface molecules. Later, they migrate to the periphery as immunocompetent T cells expressing clonally distributed TCR structures (1-4). Four different TCR genes (a, a, y, and S) have thus far been identified and shown to be specifically rearranged and expressed throughout intrathymic T cell development (5-13) . They code for two distinct types of heterodimericTCR: the common MHC-restricted a/(3 heterodimer expressed on most functional T lymphocytes (14-16), and the recently described y/8 TCRcomplex, expressed on a minor T cell subset (17-19). Both structures are expressed in association with the monomorphic CD3 (T3) complex, but they seem to be acquired independently by distinct intrathymic subpopulations (20, 21) . Developmental studies in mice support that the TCRy/S appears first in ontogeny on early double-negative (CD4- CD8- ) thymocytes. Further maturation leads to a gradual decrease of y/8-bearing cells . In contrast, TCRa/Q expression increases throughout T cell ontogeny concomitantly with the acquisition ofCD4 and/or CD8 molecules by mature T cells, expression of TCRy/S being restricted to a small population of CD4" CD8- adult thymocytes and peripheral T cells (3, 4) . These findings suggest that y/8-bearing CD4- CD8- cells may define a separate T cell lineage whose intrathymic development precedes that of classical a/a mature T cells (21, 22). Nonetheless, the presence of y gene rearrangements in mature a/0-bearing T cells (23), as well as the finding of partial a gene rearrangements in TCRy/S+ cells (22), indicate that both T cell lineages may derive from a common precursor (24). At present, however, the regulatory mechanisms underlying these developmental processes remain poorly understood, and precursor-product relationships involving the various intrathymic subpopulations continue to be disputed, making it difficult to establish direct correlations between the described patterns of TCR gene expression and a functional pathway of T cell development. Here, in vitro differentiation approaches were used to analyze the precursor potential and the putative progeny of a minor population of adult human thymocytes that lack conventional T cell markers (CD2-1-3-4-8- ; i.e., Tll - 6-3- 4- 8-) but express CD45 (i.e., T200) and CD7 molecules, suggesting that they are the most immature intrathymic progenitors (25) . Moreover, only y chain functional RNA messages are expressed in this subset, whereas a and Q chain TCR genes remain in germline configuration . Interestingly enough, in vitro culture of this subpopulation in the presence of IL-2 led to an extensive cellular proliferation and the concomitant differentiation into both TCR-y/S` and TCR-a/(3 + thymic subsets . These data support the involvement of the IL-2 pathway in the intrathymic maturation of early T cell precursors. Furthermore, they provide a useful in vitro system to induce expression of TCR-a/(3 as well asTCRy/S structures in developing thymocytes, making it feasible to investigate the cellular and molecular basis for T cell repertoire selection and development operating in T cell differentiation .-
dc.descriptionPeer reviewed-
dc.format1445620 bytes-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherRockefeller University Press-
dc.rightsopenAccess-
dc.titleInvolvement of the interleukin 2 pathway in the rearrangement and expression of both [alfa/beta] and [gamma/delta] T cell receptor genes in human T cell precursors-
dc.typeArtículo-
Appears in Collections:Digital Csic

Files in This Item:
There are no files associated with this item.
Show simple item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.