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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Martínez-Abarca, Francisco | - |
| dc.creator | Toro, Nicolás | - |
| dc.date | 2008-04-23T10:19:48Z | - |
| dc.date | 2008-04-23T10:19:48Z | - |
| dc.date | 2000 | - |
| dc.date.accessioned | 2017-01-31T01:03:09Z | - |
| dc.date.available | 2017-01-31T01:03:09Z | - |
| dc.identifier | Nucleic Acids Research, 2000 November 1; 28(21): 4397–4402 | - |
| dc.identifier | 0305-1048 | - |
| dc.identifier | http://hdl.handle.net/10261/3691 | - |
| dc.identifier | 10.1093/nar/28.21.4397 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3691 | - |
| dc.description | Final full-text version of the paper available at: http://nar.oxfordjournals.org/cgi/content/abstract/28/21/4397 .-- Copyright © by Oxford University Press. -- Http://nar.oxfordjournals.org/ | - |
| dc.description | RmInt1 is a group II intron of Sinorhizobium meliloti which was initially found within the insertion sequence ISRm2011-2. Although the RmInt1 intron-encoded protein lacks a recognizable endonuclease domain, it is able to mediate insertion of RmInt1 at an intron-specific location in intronless ISRm2011-2 recipient DNA, a phenomenon termed homing. Here we have characterized three additional insertion sites of RmInt1 in the genome of S.meliloti. Two of these sites are within IS elements closely related to ISRm2011-2, which appear to form a characteristic group within the IS630-Tc1 family. The third site is in the oxi1 gene, which encodes a putative oxide reductase. The newly identified integration sites contain conserved intron-binding site (IBS1 and IBS2) and δ′ sequences (14 bp). The RNA of the intron-containing oxi1 gene is able to splice and the oxi1 site is a DNA target for RmInt1 transposition in vivo. Ectopic transposition of RmInt1 into the oxi1 gene occurs at 20-fold lower efficiency than into the homing site (ISRm2011-2) and is independent of the major RecA recombination pathway. The possibility that transposition of RmInt1 to the oxi1 site occurs by reverse splicing into DNA is discussed. | - |
| dc.description | This work was supported by grant BIO99-0905 from the Comisión Asesora de Investigación Científica y Técnica and by grant BIO4-CT98-0483 from the Biotechnology Programme of the EU. | - |
| dc.description | Peer reviewed | - |
| dc.format | 22195 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Oxford University Press | - |
| dc.rights | openAccess | - |
| dc.title | RecA-independent ectopic transposition in vivo of a bacterial group II intron | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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