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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3691| Title: | RecA-independent ectopic transposition in vivo of a bacterial group II intron |
| Publisher: | Oxford University Press |
| Description: | Final full-text version of the paper available at: http://nar.oxfordjournals.org/cgi/content/abstract/28/21/4397 .-- Copyright © by Oxford University Press. -- Http://nar.oxfordjournals.org/ RmInt1 is a group II intron of Sinorhizobium meliloti which was initially found within the insertion sequence ISRm2011-2. Although the RmInt1 intron-encoded protein lacks a recognizable endonuclease domain, it is able to mediate insertion of RmInt1 at an intron-specific location in intronless ISRm2011-2 recipient DNA, a phenomenon termed homing. Here we have characterized three additional insertion sites of RmInt1 in the genome of S.meliloti. Two of these sites are within IS elements closely related to ISRm2011-2, which appear to form a characteristic group within the IS630-Tc1 family. The third site is in the oxi1 gene, which encodes a putative oxide reductase. The newly identified integration sites contain conserved intron-binding site (IBS1 and IBS2) and δ′ sequences (14 bp). The RNA of the intron-containing oxi1 gene is able to splice and the oxi1 site is a DNA target for RmInt1 transposition in vivo. Ectopic transposition of RmInt1 into the oxi1 gene occurs at 20-fold lower efficiency than into the homing site (ISRm2011-2) and is independent of the major RecA recombination pathway. The possibility that transposition of RmInt1 to the oxi1 site occurs by reverse splicing into DNA is discussed. This work was supported by grant BIO99-0905 from the Comisión Asesora de Investigación Científica y Técnica and by grant BIO4-CT98-0483 from the Biotechnology Programme of the EU. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3691 |
| Other Identifiers: | Nucleic Acids Research, 2000 November 1; 28(21): 4397–4402 0305-1048 http://hdl.handle.net/10261/3691 10.1093/nar/28.21.4397 |
| Appears in Collections: | Digital Csic |
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