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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Sánchez, María José | - |
| dc.creator | Gutiérrez Ramos, José Carlos | - |
| dc.creator | Fernández, Edgar | - |
| dc.creator | Leonardo, Esther | - |
| dc.creator | Lozano, Juan José | - |
| dc.creator | Martínez-Alonso, Carlos | - |
| dc.creator | Toribio, María Luisa | - |
| dc.date | 2008-04-23T12:17:21Z | - |
| dc.date | 2008-04-23T12:17:21Z | - |
| dc.date | 1993-01 | - |
| dc.date.accessioned | 2017-01-31T01:03:14Z | - |
| dc.date.available | 2017-01-31T01:03:14Z | - |
| dc.identifier | The Journal of Experimental Medicine, Volume 177, January 1993, pp. 19-33 | - |
| dc.identifier | 0022-1007 | - |
| dc.identifier | http://hdl.handle.net/10261/3699 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3699 | - |
| dc.description | El copyright pertenece a The Rockefeller University Press | - |
| dc.description | Hematopoietic cells present in the liver in early human fetal life were characterized by phenotypic analysis using a broad panel of monoclonal antibodies. Expression of very late antigen 4 and leukocyte function-associated antigen 3 cell adhesion receptors and 4F2 cell activation molecules was found in all fetal liver hematopoietic cells before acquisition of T cell-, B cell-, or myeloidspecific surface markers, and before the time ofintrathymic colonization. Molecular studies showed that expression of the interleukin 2 receptor [beta] (IL-2R[Beta]) also occurred in the embryonic liver at this early ontogenic stage. In contrast, no expression of IL-2R[alfa] or IL-2 transcripts was found in fetal liver cells, whereas transcription of the IL-4 gene was detected in a small fetal liver cell subset. Putative T cell precursors were identified among the hematopoietic fetal liver cells by the expression of genes encoding the [gamma,delta,epsilon] and [zera] invariant chains of the CD3-T cell receptor (TCR) complex. However, no transcription of the polymorphic [alfa] and [beta] TCR genes was detected. Functional in vitro assays further demonstrated that fetal liver hematopoietic cells from those early embryos were capable of proliferating in response to T cell growth factors, including IL-4 and IL-2. However, whereas IL-4-induced proliferation paralleled the appearance in vitro of CD45+CD7-CD4 'hm cells expressing the CD14 myeloid antigen, as well as of CD34 + primitive hematopoietic progenitors, differentiation into CD45 + CD7 + CD8 + CD3- immature T cells was observed when using II.-2. Moreover, coculture with thymic epithelial cell monolayers provided additional evidence that early fetal liver hematopoietic cells may include very primitive T cell precursors, which were able to differentiate in vitro into TCR [alfa/beta] + mature T cells. Therefore, our results indicate that, after triggering of the T cell-specific maturation program in primitive fetal liver hematopoietic progenitors, specific signals provided intrathymically by epithelial cells may fulfill the requirements to drive terminal differentiation of prethymically committed T cell precursors. | - |
| dc.description | Peer reviewed | - |
| dc.format | 2490557 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Rockefeller University Press | - |
| dc.rights | openAccess | - |
| dc.title | Putative prethymic T cell precursors within the early human embryonic liver: a molecular and functional analysis | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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