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dc.creatorYruela Guerrero, Inmaculada-
dc.creatorTorrado Plou, Elena-
dc.creatorRoncel Gil, Mercedes-
dc.creatorPicorel Castaño, Rafael-
dc.date2008-04-30T14:10:51Z-
dc.date2008-04-30T14:10:51Z-
dc.date2001-03-
dc.date.accessioned2017-01-31T01:06:38Z-
dc.date.available2017-01-31T01:06:38Z-
dc.identifierPhotosynthesis Research 67(3): 199-206, 2001-
dc.identifier0166-8595 (print version)-
dc.identifier1573-5079 (electronic version)-
dc.identifierhttp://hdl.handle.net/10261/3956-
dc.identifier10.1023/A:1010682416016-
dc.identifier.urihttp://dspace.mediu.edu.my:8181/xmlui/handle/10261/3956-
dc.descriptionThe definitive version is available at: http://www.springerlink.com/content/l304h18744r52202/fulltext.html-
dc.descriptionThe light-induced difference absorption spectra associated to the photo-accumulation of reduced pheophytin a were studied in the isolated D1–D2–Cyt b559 complex in the presence of variable methyl viologen concentrations and different illumination conditions under anaerobiosis. Depending on the methyl viologen/reaction centre ratio, the relative intensities of the spectral bands at 681.5±0.5, 667.0±0.5 and 542.5±0.5 nm were modified. The reduced pheophytin a located at the D1-branch of the complex absorbs at 681.7±0.5 nm, and at least two additional pigment species contribute to the Qy band of the difference absorption spectra with maxima at 667.0±0.5 and 680.5±0.5 nm. We propose the additional species correspond to a peripheral chlorophyll a and the pheophytin a located at the D2-branch of the complex, respectively. The blue absorbing chlorophyll at 667 nm is susceptible to chemical redox changes with a midpoint reduction potential of +470 mV. The Qx absorption bands of both pheophytins localised at the D2- and D1-branch of the D1–D2–Cyt b559 complex were at 540.7±0.5 and 542.9±0.5, respectively. The results indicated that the two pheophytin molecules can be photoreduced in the D1–D2–Cyt b559 complex in certain experimental conditions.-
dc.descriptionThis work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB98-1632).-
dc.descriptionPeer reviewed-
dc.format321506 bytes-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSpringer-
dc.rightsopenAccess-
dc.subjectAbsorption-
dc.subjectChlorophyll-
dc.subjectPheophytin-
dc.subjectPhotosystem II-
dc.subjectReaction centre-
dc.subjectSpectroscopy-
dc.titleLight-induced absorption spectra of the D1-D2-cytochrome b559 complex of the Photosystem II: Effect of methyl viologen concentration-
dc.typeArtículo-
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