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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Abdu, Uri | - |
| dc.creator | González-Reyes, Acaimo | - |
| dc.creator | Ghabrial, Amin | - |
| dc.creator | Schüpbach, Trudi | - |
| dc.date | 2008-05-06T16:09:41Z | - |
| dc.date | 2008-05-06T16:09:41Z | - |
| dc.date | 2003-09 | - |
| dc.date.accessioned | 2017-01-31T01:11:02Z | - |
| dc.date.available | 2017-01-31T01:11:02Z | - |
| dc.identifier | Genetics 165: 197–204 (September 2003). | - |
| dc.identifier | http://www.genetics.org/cgi/content/abstract/165/1/197 | - |
| dc.identifier | 0016-6731 | - |
| dc.identifier | http://hdl.handle.net/10261/4048 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4048 | - |
| dc.description | Sequence data from this article have been deposited with the Gen-Bank data libraries under accession no. AY257540. | - |
| dc.description | In Drosophila, mutations in double-strand DNA break (DSB) repair enzymes, such as spn-B, activate a meiotic checkpoint leading to dorsal-ventral patterning defects in the egg and an abnormal appearance of the oocyte nucleus. Mutations in spn-D cause an array of ovarian phenotypes similar to spn-B. We have cloned the spn-D locus and found that it encodes a protein of 271 amino acids that shows significant homology to the human RAD51C protein. In mammals the spn-B and spn-D homologs, XRCC3 and RAD51C, play a role in genomic stability in somatic cells. To test for a similar role for spn-B and spn-D in double-strand DNA repair in mitotic cells, we analyzed the sensitivity of single and double mutants to DSBs induced by exposure to X rays and MMS. We found that neither singly mutant nor doubly mutant animals were significantly sensitized to MMS or X rays. These results suggest that spn-B and spn-D act in meiotic recombination but not in repair of DSBs in somatic cells. As there is no apparent ortholog of the meiosis-specific DMC1 gene in the Drosophila genome, and given their meiosis-specific requirement, we suggest that spn-B and spn-D may have a function comparable to DMC1. | - |
| dc.description | This work was supported by the PHS Grant PO1CA41086 and by the Howard Hughes Medical Institute. A.G.-R. acknowledges the financial support of the Spanish Ministerio de Ciencia y Tecnología (grant nos. PM99-0107 and BMC2001-4822-E), the Junta de Andalucía (CVI 724), and the EMBO Young Investigator Programme. | - |
| dc.description | Peer reviewed | - |
| dc.format | 229365 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Genetics Society of America | - |
| dc.rights | openAccess | - |
| dc.subject | Mutations | - |
| dc.subject | Drosophila | - |
| dc.subject | DNA breaks | - |
| dc.subject | Repair enzymes | - |
| dc.subject | Recombination | - |
| dc.subject | Meiosis | - |
| dc.title | The Drosophila spn-D gene encodes a RAD51C-like protein that is required exclusively during meiosis | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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