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Please use this identifier to cite or link to this item: http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4095
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dc.creatorGarcía-Rubio, María L.-
dc.creatorChávez, Sebastián-
dc.creatorHuertas Sánchez, Pablo-
dc.creatorTous, Cristina-
dc.creatorJimeno, Sonia-
dc.creatorLuna, Rosa-
dc.creatorAguilera, Andrés-
dc.date2008-05-08T15:29:56Z-
dc.date2008-05-08T15:29:56Z-
dc.date2008-02-
dc.date.accessioned2017-01-31T01:12:34Z-
dc.date.available2017-01-31T01:12:34Z-
dc.identifierMolecular Genetics and Genomics 279(2): 123–132 (2008)-
dc.identifier1617-4615-
dc.identifierhttp://hdl.handle.net/10261/4095-
dc.identifier10.1007/s00438-007-0301-6-
dc.identifier1617-4623-
dc.identifier.urihttp://dspace.mediu.edu.my:8181/xmlui/handle/10261/4095-
dc.descriptionPMID: 17960421.-- Final full-text version available at: http://dx.doi.org/10.1007/s00438-007-0301-6 (www.springerlink.com)-
dc.descriptionTHO/TREX is a conserved nuclear complex that functions in mRNP biogenesis and plays a role in preventing the transcription-associated genetic instability. THO is composed of Tho2, Hpr1, Mft1 and Thp2 subunits, which associate with the Sub2-Yra1 export factors and Tex1 to form the TREX complex. To compare the functional relevance of the different THO/TREX subunits, we determined the effect of their null mutations on mRNA accumulation and recombination. Unexpectedly, we noticed that a full deletion of HPR1, hpr1DeltaK, conferred stronger hyper-recombination phenotype and gene expression defects than did hpr1DeltaH, the allele encoding a C-terminal truncated protein which was used in most previous studies. We show that tho2Delta and, to a lesser extent, hpr1DeltaK are the THO mutations with the highest impact on all phenotypes, and that sub2Delta shows a similar transcription-dependent hyper-recombination phenotype and in vivo transcription impairment as hpr1DeltaK and tho2Delta. Recombination and transcription analyses indicate that THO/TREX mutants share a moderate but significant effect on gene conversion and ectopic recombination, as well as transcription impairment of even short and low GC-content genes. Our data provide new information on the relevance of these proteins in mRNP biogenesis and in the maintenance of genomic integrity.-
dc.descriptionThis work was supported by the Ministry of Science and Education of Spain (grants SAF2003–00204 and BMC2006–05260) and Junta de Andalucía (CVI-102).-
dc.descriptionPeer reviewed-
dc.format22195 bytes-
dc.formatapplication/pdf-
dc.languageeng-
dc.publisherSpringer-
dc.relationhttp://dx.doi.org/10.1007/s00438-007-0301-6-
dc.rightsclosedAccess-
dc.subjectTHO complex-
dc.subjectSub2-
dc.subjectGenetic instability-
dc.subjectmRNP biogenesis-
dc.subjectTranscription-
dc.subjectTranscription-associated recombination-
dc.titleDifferent physiological relevance of yeast THO/TREX subunits in gene expression and genome integrity-
dc.typeArtículo-
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