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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4096Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Tous, Cristina | - |
| dc.creator | Aguilera, Andrés | - |
| dc.date | 2008-05-08T15:46:25Z | - |
| dc.date | 2008-05-08T15:46:25Z | - |
| dc.date | 2007-08-24 | - |
| dc.date.accessioned | 2017-01-31T01:12:37Z | - |
| dc.date.available | 2017-01-31T01:12:37Z | - |
| dc.identifier | Biochem Biophys Res Commun. 360(2): 428-432 (2007). | - |
| dc.identifier | 0006-291X | - |
| dc.identifier | http://hdl.handle.net/10261/4096 | - |
| dc.identifier | 10.1016/j.bbrc.2007.06.098 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4096 | - |
| dc.description | PMID: 17603014.-- Final version of the paper available at: http://www.sciencedirect.com/science/journal/0006291X | - |
| dc.description | Transcription elongation causes a local change in DNA superhelicity. An excess of negative supercoiling may lead to opening of DNA strands that could allow formation of R-loops. In yeast, mutants of the THO complex are impaired in transcription elongation and this defect has been linked to co-transcriptional formation of R-loops, which could constitute roadblocks for RNA polymerases. In this study, we found that stably formed 300-nt long DNA–RNA hybrids in a negatively supercoiled transcription template reduced the efficiency of transcription elongation by half, providing a first experimental evidence that transcription elongation is impaired by R-loops in vitro. | - |
| dc.description | This work was supported by grants of the Spanish Ministry of Science and Education (GEN2001-4707-C08-02 and SAF2003-00204) and Junta de Andalucía (CVI102). | - |
| dc.description | Peer reviewed | - |
| dc.format | 22195 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | http://dx.doi.org/10.1016/j.bbrc.2007.06.098 | - |
| dc.rights | closedAccess | - |
| dc.subject | Saccharomyces cerevisiae | - |
| dc.subject | R-loops | - |
| dc.subject | Transcription elongation | - |
| dc.subject | In vitro transcription | - |
| dc.subject | RNA polymerase II | - |
| dc.subject | DNA–RNA hybrid | - |
| dc.subject | RNA | - |
| dc.title | Impairment of transcription elongation by R-loops in vitro | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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