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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4148Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.creator | Yruela Guerrero, Inmaculada | - |
| dc.creator | Allakhverdiev, Suleyman I. | - |
| dc.creator | Ibarra, José V. | - |
| dc.creator | Klimov, Vyacheslav V. | - |
| dc.date | 2008-05-12T10:32:01Z | - |
| dc.date | 2008-05-12T10:32:01Z | - |
| dc.date | 1998-04 | - |
| dc.date.accessioned | 2017-01-31T01:14:01Z | - |
| dc.date.available | 2017-01-31T01:14:01Z | - |
| dc.identifier | FEBS Lett 425 (3): 396-400, 1998 | - |
| dc.identifier | http://hdl.handle.net/10261/4148 | - |
| dc.identifier | 10.1016/S0014-5793(98)00271-3 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4148 | - |
| dc.description | The definitive version is available at http://www.febsletters.org/article/S0014-5793(98)00271-3/ | - |
| dc.description | The light-induced Fourier transform infrared difference (FT-IR) spectrum originating from the donor side of O2-evolving photosystem (PS) II was obtained in non-depleted and CO2-depleted PSII membrane preparations. The observed spectrum free of contributions from the acceptor side signals was achieved by employing 2 mM/18 mM ferri-/ferrocyanide as a redox couple. This spectrum showed main positive bands at 1589 and 1365 cm−1 and negative bands at 1560, 1541, 1522 and 1507 cm−1. CO-depleted PSII preparations showed a quite different spectrum. The main positive and negative bands disappeared after depletion of bicarbonate. The addition of bicarbonate partially restored those bands again. Comparison between difference FT-IR spectra of untreated and bicarbonate-depleted PSII membranes indicated that the positive bands at 1589 and 1365 cm−1 can be assigned to COO− stretching modes from bicarbonate. The higher frequency corresponds to uas (COO−) and the lower frequency to us (COO−). 13C-Labeling FT-IR measurements confirmed these findings and also suggested that the negative band at 1560 cm−1 can be ascribed to uas (COO−). The data are discussed in the framework of the suggestion that bicarbonate can be a ligand to the Mn-containing water-oxidizing complex of PSII. | - |
| dc.description | This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB 95-0219), by Sabbatical Fellowships to V.V.K. and S.I.A. from the Spanish Ministry of Education and Culture and by the Russian Foundation of Basic Research (Grant 96-04-50394). | - |
| dc.description | Peer reviewed | - |
| dc.format | 3313625 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.rights | openAccess | - |
| dc.subject | Photosystem II | - |
| dc.subject | Bicarbonate | - |
| dc.subject | Donor side | - |
| dc.subject | Fourier transform | - |
| dc.subject | Infrared spectroscopy | - |
| dc.title | Bicarbonate binding to the water-oxidizing complex in the photosystem II. A Fourier transform infrared spectroscopy study | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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