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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Yruela Guerrero, Inmaculada | - |
| dc.creator | Tomás Corruchaga, Raquel | - |
| dc.creator | Sanjuán, M. L. | - |
| dc.creator | Torrado Plou, Elena | - |
| dc.creator | Aured Galve, María | - |
| dc.creator | Picorel Castaño, Rafael | - |
| dc.date | 2008-05-12T12:32:52Z | - |
| dc.date | 2008-05-12T12:32:52Z | - |
| dc.date | 1998-11 | - |
| dc.date.accessioned | 2017-01-31T01:14:34Z | - |
| dc.date.available | 2017-01-31T01:14:34Z | - |
| dc.identifier | Photochemistry and Photobiology 68(5): 725-737 | - |
| dc.identifier | 0031-8655 | - |
| dc.identifier | http://hdl.handle.net/10261/4166 | - |
| dc.identifier | 10.1111/j.1751-1097.1998.tb02537.x | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4166 | - |
| dc.description | The definitive version is available at http://www.blackwell-synergy.com/ | - |
| dc.description | Different pigment extraction procedures and HPLC methods were tested to investigate the geometric configuration of the β-carotene in two forms of the photosystem II reaction center (Dl-D2-cytochrome (Cyt) b559) complex containing one and two β-carotene molecules per two pheophytin a. All the handling steps and HPLC analyses were done in darkness at room temperature and at 4°C. Two different pigment extracts were analyzed, a mixture of chlorophyll a, pheophytin a and 3-carotene, and the isolated p-carotene from that mixture. In both cases only the all-frans-β-carotene was detected. The chromatographic profiles were similar at both temperatures only differing in the retention times that were longer at 4°C. This result was independent of the concentration of photosynthetic starting material. Furthermore, no differences were observed between Dl-D2-Cyt b559 complexes with one and two p-carotene molecules per reaction center. The analysis of the β-carotene chromatographic peak indicated no 15-cis to all-fraws isom-erization occurred during the HPLC chromatography in our experimental conditions. Resonance Raman spectra were also recorded in the isolated Dl-D2-Cyt b559 complex at room and liquid nitrogen temperature with excitation at 514.5 nm from an Ar+ laser. Spectra of control preparations showed main bands at 1532, 1264, 1213, 1185, 1154 and 1003 cm−1 corresponding to the M-trans isomer and confirm previous results. The presence in the reaction center suspension of artificial electron acceptors such as silicomolybdate or 2,5-dibromo-3-methyl-6-iso-propyl-p-benzoquinone that are able to quench 3P680 did not modify the resonance Raman spectra of the native Dl-D2-Cyt b559 complex. The results suggest that no isomerization takes place during the laser irradiation. | - |
| dc.description | This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB95-0219) to R.P. | - |
| dc.description | Peer reviewed | - |
| dc.format | 1468673 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Blackwell Publishing | - |
| dc.rights | openAccess | - |
| dc.subject | photosystem II | - |
| dc.subject | reaction center | - |
| dc.subject | beta-caroteno | - |
| dc.subject | HPLC | - |
| dc.subject | resonance raman | - |
| dc.title | The configuration of beta-carotene in the photosystem II reaction center | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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