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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4693| Title: | A simple and efficient method to identify replacements of P-lacZ by P-Gal4 lines allows obtaining Gal4 insertions in the bithorax complex of Drosophila |
| Authors: | Ministerio de Educación y Ciencia (España) Comunidad de Madrid Fundación Ramón Areces |
| Keywords: | Drosophila P-element Bithorax complex Gal4 lines Ubx Abd-B |
| Publisher: | Elsevier |
| Description: | The functional replacement of one gene product by another one is a powerful method to study specificity in development and evolution. In Drosophila, the Gal4/UAS method has been used to analyze in vivo such functional substitutions. To this aim, Gal4 lines that inactivate a gene and reproduce its expression pattern are required, and they can be frequently obtained by replacing pre-existing P-lacZ lines with such characteristics. We have devised a new method to quickly identify replacements of P-lacZ lines by P-Gal4 lines, and applied it successfully to obtain Gal4 insertions in the Ultrabithorax and Abdominal-B Hox genes. We have used these lines to study the functional replacement of a Hox gene by another one. Our experiments confirm that the abdominal-A gene can replace Ultrabithorax in haltere development but that it cannot substitute for Abdominal-B in the formation of the genitalia. This work was supported by grants from the Ministerio de Educación y Ciencia (BMC2002-00300), the Comunidad Autónoma de Madrid (No. 08.1/0031/2001.1 and GR/SAL/0147/2004) and an Institutional Grant from the Fundación Ramón Areces. Peer reviewed |
| URI: | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4693 |
| Other Identifiers: | Mechanisms of Development, Volume 123, Issue 11, November 2006, Pages 860-867 1872-6356 (Online) 0925-4773 (Print) http://hdl.handle.net/10261/4693 10.1016/j.mod.2006.07.010 |
| Appears in Collections: | Digital Csic |
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