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http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4921Full metadata record
| DC Field | Value | Language |
|---|---|---|
| dc.contributor | Ministerio de Educación y Ciencia (España) | - |
| dc.contributor | Wellcome Trust | - |
| dc.contributor | Fundación Ramón Areces | - |
| dc.creator | Granja, Aitor G. | - |
| dc.creator | Nogal París, María Luisa | - |
| dc.creator | Hurtado, Carolina | - |
| dc.creator | Aguila, Carmen del | - |
| dc.creator | Carrascosa, Ángel L. | - |
| dc.creator | Salas, María Luisa | - |
| dc.creator | Fresno, Manuel | - |
| dc.creator | Revilla Novella, Yolanda | - |
| dc.date | 2008-06-09T14:13:57Z | - |
| dc.date | 2008-06-09T14:13:57Z | - |
| dc.date | 2006-01 | - |
| dc.date.accessioned | 2017-01-31T01:37:10Z | - |
| dc.date.available | 2017-01-31T01:37:10Z | - |
| dc.identifier | The Journal of Immunology, 2006, 176: 451-462 | - |
| dc.identifier | 0022-1767 (Print) | - |
| dc.identifier | 1550-6606 (Online) | - |
| dc.identifier | http://hdl.handle.net/10261/4921 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/4921 | - |
| dc.description | Article available at http://www.jimmunol.org/cgi/content/abstract/176/1/451 | - |
| dc.description | African swine fever virus (ASFV) is able to inhibit TNF--induced gene expression through the synthesis of A238L protein. This was shown by the use of deletion mutants lacking the A238L gene from the Vero cell-adapted Ba71V ASFV strain and from the virulent isolate E70. To further analyze the molecular mechanism by which the viral gene controls TNF-, we have used Jurkat cells stably transfected with the viral gene to identify the TNF- regulatory elements involved in the induction of the gene after stimulation with PMA and calcium ionophore. We have thus identified the cAMP-responsive element and 3 sites on the TNF- promoter as the responsible of the gene activation, and demonstrate that A238L inhibits TNF- expression through these DNA binding sites. This inhibition was partially reverted by overexpression of the transcriptional factors NF-AT, NF-B, and c-Jun. Furthermore, we present evidence that A238L inhibits the activation of TNF- by modulating NF-B, NF-AT, and c-Jun trans activation through a mechanism that involves CREB binding protein/p300 function, because overexpression of these transcriptional coactivators recovers TNF- promoter activity. In addition, we show that A238L is a nuclear protein that binds to the cyclic AMP-responsive element/3 complex, thus displacing the CREB binding protein/p300 coactivators. Taken together, these results establish a novel mechanism in the control of TNF- gene expression by a viral protein that could represent an efficient strategy used by ASFV to evade the innate immune response | - |
| dc.description | This work was supported by grants from Ministerio de Educación y Ciencia (BFU2004-00298/BMC), the Wellcome Trust (075813/C/04/z), and by an institutional grant from the Fundación Ramón Areces. C.H. was a fellow from Fundación Ramón Areces. | - |
| dc.description | Peer reviewed | - |
| dc.format | 3436629 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Association of Immunologists | - |
| dc.rights | openAccess | - |
| dc.subject | ASFV | - |
| dc.subject | A238L protein | - |
| dc.title | The Viral Protein A238L Inhibits TNF-α Expression through a CBP/p300 Transcriptional Coactivators Pathway | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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