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| DC Field | Value | Language |
|---|---|---|
| dc.creator | Agudo-Ibáñez, Lorena | - |
| dc.creator | Núñez, Fátima | - |
| dc.creator | Calvo, Fernando | - |
| dc.creator | Berenjeno, Inmaculada M. | - |
| dc.creator | Bustelo, Xosé R. | - |
| dc.creator | Crespo, Piero | - |
| dc.date | 2008-06-17T06:52:42Z | - |
| dc.date | 2008-06-17T06:52:42Z | - |
| dc.date | 2007-11 | - |
| dc.date.accessioned | 2017-01-31T01:42:11Z | - |
| dc.date.available | 2017-01-31T01:42:11Z | - |
| dc.identifier | Cellular Signalling 19(11): 2264–2276 (2007) | - |
| dc.identifier | 0898-6568 | - |
| dc.identifier | http://hdl.handle.net/10261/5124 | - |
| dc.identifier | 10.1016/j.cellsig.2007.06.025 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5124 | - |
| dc.description | El pdf del artículo es el manuscrito de autor. | - |
| dc.description | Ras proteins are distributed in distinct plasma-membrane microdomains and endomembranes. The biochemical signals generated by Ras therein differ qualitatively and quantitatively, but the extent to which this spatial variability impacts on the genetic program switched-on by Ras is unknown. We have used microarray technology to identify the transcriptional targets of localization-specific Ras subsignals in NIH3T3 cells expressing H-RasV12 selectively tethered to distinct cellular microenvironments. We report that the transcriptomes resulting from site-specific Ras activation show a significant overlap. However, distinct genetic signatures can also be found for each of the Ras subsignals. Our analyses unveil 121 genes uniquely regulated by Ras signals emanating from plasma-membrane microdomains. Interestingly, not a single gene is specifically controlled by lipid raft-anchored Ras. Furthermore, only 9 genes are exclusive for Ras signals from endomembranes. Also, we have identified 31 genes common to the site-specific Ras subsignals capable of inducing cellular transformation. Among these are the genes coding for Vitamin D receptor and for p120-GAP and we have assessed their impact in Ras-induced transformation. Overall, this report reveals the complexity and variability of the different genetic programs orchestrated by Ras from its main sublocalizations. | - |
| dc.description | PC’s work is supported by grants from the Spanish Ministry of Education and Science (MES) (BFU2005-00777 and GEN2003-20239-C06-03), the EU Sixth Framework Program under the SIMAP project, and the Red Temática de Investigación Cooperativa en Cáncer (RTICC) (RD06/0020/0105). Fondo de Investigaciones Sanitarias (FIS), Carlos III Institute, Spanish Ministry of Health. XRB’s work is supported by grants from the US National Cancer Institute/NIH (5R01-CA73735-10), the MES (SAF2006-01789 and GEN2003-20239-C06-01), the Castilla-León Autonomous Government (SA053A05), and the RTICC (RD06/0020/0001). F.N. was partially supported by a fellowship by the Ernst Schering Foundation. LA, FC, and IMB are Spanish Ministry of Education predoctoral fellows. All Spanish funding is co-sponsored by the European Union. | - |
| dc.description | Peer reviewed | - |
| dc.format | 2193886 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | Elsevier | - |
| dc.relation | http://dx.doi.org/10.1016/j.cellsig.2007.06.025 | - |
| dc.rights | openAccess | - |
| dc.subject | Ras | - |
| dc.subject | Compartmentalization | - |
| dc.subject | Gene microarrays | - |
| dc.subject | Transformation | - |
| dc.title | Transcriptomal profiling of site-specific Ras signals | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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