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| DC Field | Value | Language |
|---|---|---|
| dc.creator | López-Enríquez, Lorena | - |
| dc.creator | Rodríguez-Lázaro, David | - |
| dc.creator | Hernández, Marta | - |
| dc.date | 2008-06-20T07:50:15Z | - |
| dc.date | 2008-06-20T07:50:15Z | - |
| dc.date | 2007-06 | - |
| dc.date.accessioned | 2017-01-31T01:44:28Z | - |
| dc.date.available | 2017-01-31T01:44:28Z | - |
| dc.identifier | Applied and Environmental Microbiology 73(11): 3747–3751 (2007) | - |
| dc.identifier | 0099-2240 | - |
| dc.identifier | http://hdl.handle.net/10261/5200 | - |
| dc.identifier | 10.1128/AEM.02642-06 | - |
| dc.identifier.uri | http://dspace.mediu.edu.my:8181/xmlui/handle/10261/5200 | - |
| dc.description | We developed a real-time PCR assay for the quantitative detection of Clostridium tyrobutyricum, which has been identified as the major causal agent of late blowing in cheese. The assay was 100% specific, with an analytical sensitivity of 1 genome equivalent in 40% of the reactions. The quantification was linear (R2 > 0.9995) over a 5-log dynamic range, down to 10 genome equivalents, with a PCR efficiency of >0.946. With optimized detergent treatment and enzymatic pretreatment of the sample before centrifugation and nucleic acid extraction, the assay counted down to 300 C. tyrobutyricum spores, with a relative accuracy of 82.98 to 107.68, and detected as few as 25 spores in 25 ml of artificially contaminated raw or ultrahigh-temperature-treated whole milk. | - |
| dc.description | This work was supported by the Agrarian Experimental Plan of the ITACyL/Junta de Castilla y León and the European Union's Marie-Curie Mobility Program (contract MEIF-CT-2005-0011564). L.L.-E. received a Ph.D. studentship from the Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA), D.R.-L. is a fellow of the European Union's Marie-Curie Mobility Program, and M.H. holds a contract from the INIA. | - |
| dc.description | Peer reviewed | - |
| dc.format | 25095 bytes | - |
| dc.format | application/pdf | - |
| dc.language | eng | - |
| dc.publisher | American Society for Microbiology | - |
| dc.relation | http://dx.doi.org/10.1128/AEM.02642-06 | - |
| dc.rights | openAccess | - |
| dc.title | Quantitative detection of clostridium tyrobutyricum in milk by real-time PCR | - |
| dc.type | Artículo | - |
| Appears in Collections: | Digital Csic | |
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