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http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/3137| Title: | Establishment of an in vitro system for studies on the induced resistance of cotton to Xanthomonas campestris pv. malvacearum |
| Keywords: | Gossypium hirsutum bacterial blight extracellular polysaccharides cell suspension culture hypersensitive response |
| Issue Date: | 30-May-2013 |
| Publisher: | Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
| Description: | An in vitro system for studying the resistance response of cotton (Gossypium hirsutum L.) to Xanthomonas campestris pv. malvacearum was investigated. Cell suspension cultures, established from hypocotyl-derived callus of cotton cultivar 101-102B, were treated with bacterial extracellular polysaccharides (EPS) extracted from the incompatible race 18 of X. campestris pv. malvacearum. EPS at 600 mug/mL caused pronounced darkening of the suspension cultures, as indicative of cell death, 48 hours after incubation. Protein electrophoresis analysis of the time course of EPS-treated cells showed differential accumulation of several protein bands after 12-24 hours. The time course of protein accumulation and cell death was consistent with an elicitor-mediated hypersensitive response. |
| URI: | http://koha.mediu.edu.my:8181/jspui/handle/123456789/3137 |
| Other Identifiers: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2000000400007 http://www.doaj.org/doaj?func=openurl&genre=article&issn=0100204X&date=2000&volume=35&issue=4&spage=719 |
| Appears in Collections: | Agriculture and Food Sciences |
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