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http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/3935| Title: | Genetic diversity and virulence pattern in field populations of Pyricularia grisea from rice cultivar Metica-1 |
| Keywords: | Oryza sativa pathotypes rice blast pathogenicity |
| Issue Date: | 30-May-2013 |
| Publisher: | Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
| Description: | Rice blast is a major yield constraint of the irrigated rice in the State of Tocantins, Brazil. The objective of this investigation was to study the phenotypic and genetic diversity within the pathogen population of Pyricularia grisea in samples collected from four individual farms of rice cultivar Metica-1, under epidemic conditions of leaf blast. A set of 87 isolates was tested on 32 rice genotypes including eight international differentials. Considering 80% similarity in virulence, two groups comprising a total of 81 isolates were recognized, independently of the farms from which they were collected. Eighty percent of the isolates pertained to pathotype ID-14, indicating high cultivar specificity and narrow diversity of virulence in the sample population. The virulence in pathogen population on rice cultivars BR-IRGA 409 and Rio Formoso was low. Analysis of P. grisea isolates using rep-PCR with two primer sequences from Pot2 generated fingerprint profiles of one to nine bands. Cluster analysis revealed the occurrence of six fingerprint groups with similarities ranging from 0.09 to 1. There was no straight relationship between virulence of the isolates based on reaction pattern on 32 genotypes and grouping based on Pot2 rep-PCR analysis of P. grisea isolates collected from 'Metica-1'. |
| URI: | http://koha.mediu.edu.my:8181/jspui/handle/123456789/3935 |
| Other Identifiers: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2002001200002 http://www.doaj.org/doaj?func=openurl&genre=article&issn=0100204X&date=2002&volume=37&issue=12&spage=1681 |
| Appears in Collections: | Agriculture and Food Sciences |
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