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http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/4824| Title: | In Vitro and In Vivo Evaluation of a Folate-Targeted Copolymeric Submicrohydrogel Based on N-Isopropylacrylamide as 5-Fluorouracil Delivery System |
| Keywords: | drug delivery folate-conjugate nanogel 5-fluorouracil culture cells pharmacokinetic |
| Issue Date: | 30-May-2013 |
| Publisher: | Molecular Diversity Preservation International |
| Description: | Folate-targeted poly[(p-nitrophenyl acrylate)-co-(N-isopropylacrylamide)] nanohydrogel (F-SubMG) was loaded with 5-fluorouracil (5-FU) to obtain low (16.3 ± 1.9 μg 5-FU/mg F-SubMG) and high (46.8 ± 3.8 μg 5-FU/mg F-SubMG) load 5-FU-loaded F-SubMGs. The complete in vitro drug release took place in 8 h. The cytotoxicity of unloaded F-SubMGs in MCF7 and HeLa cells was low; although it increased for high F-SubMG concentration. The administration of 10 μM 5-FU by 5-FU-loaded F-SubMGs was effective on both cellular types. Cell uptake of F-SubMGs took place in both cell types, but it was higher in HeLa cells because they are folate receptor positive. After subcutaneous administration (28 mg 5-FU/kg b.w.) in Wistar rats, F-SubMGs were detected at the site of injection under the skin. Histological studies indicated that the F-SubMGs were surrounded by connective tissue, without any signs of rejections, even 60 days after injection. Pharmacokinetic study showed an increase in MRT (mean residence time) of 5-FU when the drug was administered by drug-loaded F-SubMGs. |
| URI: | http://koha.mediu.edu.my:8181/jspui/handle/123456789/4824 |
| Other Identifiers: | http://www.mdpi.com/2073-4360/3/3/1107/ http://www.doaj.org/doaj?func=openurl&genre=article&issn=20734360&date=2011&volume=3&issue=3&spage=1107 |
| Appears in Collections: | Science General |
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