Please use this identifier to cite or link to this item: http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/5029
Title: Evaluation of Origanum Vulgare L. ssp. Viridis Leaves Extract Effect on Discrimination Learning and LTP Induction in the CA1 Region of the Rat Hippocampus
Keywords: Antioxidant assay
Long term potentiation
Medicinal plant
Origanum vulgare
Spatial learning
Issue Date: 30-May-2013
Publisher: Mashhad University of Medical Sciences
Description: AbstractObjective(s)The objective of this study was to determine the effect of aqueous extract of Origanum vulgare L. ssp. Viridis (ORG) on discrimination learning and long term potentiation (LTP) in CA1 region of the rat hippocampus. Materials and MethodsA group of adult male Wistar rats weighing 27525 g received aqueous extract of ORG (150, 300, 450 mg/kg/day) by intraperitoneal injection for one week, and the other group received saline (n= 6). A wooden T-maze was used to evaluate the discrimination learning. In electrophysiological experiments, the effect of ORG leaves extract on induction and maintenance of long term potentiation (LTP) in CA1 hippocampus area was determined. LTP was evaluated in CA1 region after high-frequency stimulation (200 Hz) of the Schaffer collaterals. Also, serum antioxidant levels were analyzed in the two groups (n= 4).ResultsStatistical analysis showed significant decreases in the number of total (significantly at the dose of 300 and 450 mg/kg) and wrong (significantly at the dose of 300 mg/kg) entrance into opposite box of T-maze procedure in ORG-treated animals (P< 0.05). In electrophysiological study, the rats which had received ORG (150, 300, and 450 mg/kg) showed an increase in both population spike amplitude (59.7±14.1%, 85±14.7% and 49.3±8.7% respectively, compared to 39±9.2% increase in saline group) and maintenance of LTP in hippocampus CA1 after high frequency stimulation in Schaffer collateral pathway. In serum antioxidant assay, level of antioxidants in ORG groups (300 and 450 mg/kg) remarkably increased in comparison to saline group (P< 0.05 and P< 0.001, in turn).ConclusionOur results suggest that Origanum aqueous extract can improve the learning criteria in rats.
URI: http://koha.mediu.edu.my:8181/jspui/handle/123456789/5029
Other Identifiers: http://www.mums.ac.ir/shares/basic_medical/basicmedjou/2011/mar/a11.pdf
http://www.doaj.org/doaj?func=openurl&genre=article&issn=20083866&date=2011&volume=14&issue=49&spage=
Appears in Collections:Health Sciences

Files in This Item:
There are no files associated with this item.


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.