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http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/5627| Title: | In Vivo and in Vitro Anti-Inflammatory Activity of Neorogioltriol, a New Diterpene Extracted from the Red Algae Laurencia glandulifera |
| Keywords: | anti-inflammatory neorogioltriol Laurencia TNFα NF-κB NO COX-2 carrageenan |
| Issue Date: | 30-May-2013 |
| Publisher: | Molecular Diversity Preservation International |
| Description: | Neorogioltriol is a tricyclic brominated diterpenoid isolated from the organic extract of the red algae Laurencia glandulifera. In the present study, the anti-inflammatory effects of neorogioltriol were evaluated both in vivo using carrageenan-induced paw edema and in vitro on lipopolysaccharide (LPS)-treated Raw264.7 macrophages. The in vivo study demonstrated that the administration of 1 mg/kg of neorogioltriol resulted in the significant reduction of carregeenan-induced rat edema. In vitro, our results show that neorogioltriol treatment decreased the luciferase activity in LPS-stimulated Raw264.7 cells, stably transfected with the NF-κB-dependent luciferase reporter. This effect on NF-κB activation is not mediated through MAPK pathways. The inhibition of NF-κB activity correlates with decreased levels of LPS-induced tumor necrosis factor-alpha (TNFα) present in neorogioltriol treated supernatant cell culture. Further analyses indicated that this product also significantly inhibited the release of nitric oxide and the expression of cyclooxygenase-2 (COX-2) in LPS-stimulated Raw264.7 cells. These latter effects could only be observed for neorogioltriol concentrations below 62.5 µM. To our knowledge, this is the first report describing a molecule derived from Laurencia glandulifera with anti-inflammatory activity both in vivo and in vitro. The effect demonstrated in vitro may be explained by the inhibition of the LPS-induced NF-κB activation and TNFα production. NO release and COX-2 expression may reinforce this effect. |
| URI: | http://koha.mediu.edu.my:8181/jspui/handle/123456789/5627 |
| Other Identifiers: | http://www.mdpi.com/1660-3397/9/7/1293/ http://www.doaj.org/doaj?func=openurl&genre=article&issn=16603397&date=2011&volume=9&issue=7&spage=1293 |
| Appears in Collections: | Health Sciences |
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