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http://dspace.mediu.edu.my:8181/xmlui/handle/1957/1940| Title: | Effect of the calpain inhibitor E-64-d on the degradation of α-fodrin in damaged muscle |
| Authors: | Widrick, Jeffrey J. Wilcox, Anthony Bella, Deborah Levenson, Rick |
| Keywords: | calpain E-64-d eccentric muscle damage |
| Issue Date: | 16-Oct-2013 |
| Description: | Graduation date: 2006 We hypothesized that calpain activity is elevated in response to muscle damage. To test this hypothesis, we examined the degradation of α-fodrin into its 150 and 145 kDa fragments following either 20 eccentric or isometric contractions. In addition, experiments were performed in the presence or absence of E-64-d, a calpain inhibitor. Both EDL and SOL muscles displayed significant differences (p<0.003 and p<0.002 respectively) between the raw and normalized 150 and 145 kDa α-fodrin fragments of the DMSO + E-64-d compared to the other bath treatments. Based on our model of exercise-induced muscle damage, we expected to see greater levels of 150 and 145 kDa α-fodrin fragments in those muscles that performed the eccentric protocol. However, there was no evidence that eccentric muscle damage increased the levels of 150 and 145 kDa α-fodrin fragments over the levels observed in the isometric trials. These findings suggest that the magnitude of damage was insufficient to activate calpains. |
| URI: | http://koha.mediu.edu.my:8181/xmlui/handle/1957/1940 |
| Other Identifiers: | http://hdl.handle.net/1957/1940 |
| Appears in Collections: | ScholarsArchive@OSU |
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