http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/4043 2026-03-10T15:25:57Z 2026-03-10T15:25:57Z http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/4089 2013-05-30T10:34:29Z 2013-05-30T00:00:00Z

ISOLATION AND TAXONOMIC INVESTIGATION OF ACTINOMYCES FROM SPECIFIC BIOTOPES IN BULGARIA Investigations were performed for isolation of Actinomyces strains from different extreme biotopes in Bulgaria. According to the morphological, physiological and chemotaxonomic data three of the strains were taxonomically identified to genus, one as a member of genus Frankia and two - from genus Actinomadura . The other ten Actinomyces isolates belonged to genus Streptomyces and were also identified to species.

2013-05-30T00:00:00Z http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/4088 2013-05-30T10:33:59Z 2013-05-30T00:00:00Z

CHARACTERISTICS OF SOIL ACTINOMYCETES FROM ANTARCTICA Forty-seven actinomyces strains were isolated from Antarctic soils - nineteen of them showed antagonistic activity against Gram-positive and Gram-negative bacteria. Six of the strains possessed a broad spectrum of antibacterial activity. Results obtained from the physiological and biochemical analyses including determination of 39 characteristics proved that two of the strains (23 and 29) were similar whereas all the rest differed among each other. Morphological studies indicated that the strains belonged to the genera Streptomyces , Actinomadura and Kitasatosporia . Antibacterial activity of three actinomycetes strains (designed as 29, 30 and 47) was confirmed in batch culture. They were active against clinical isolates from the species Staphylococcus aureus and Streptococcus pneumoniae. The three strains also showed antibacterial activity against the phytopathogenic bacteria Xanthomonas axonopodis pv. glycines, X. vesicatoria, X. axonopodis pv. phaseoli, Pseudomonas syringae pv. tomato and Clavibacter michiganensis , for which no biological means for control, had been developed yet. The broadest spectrum of antibacterial action had the strain 29. The antibacterial compounds produced by these strains probably possessed non-polar structure and consisted of several active components.

2013-05-30T00:00:00Z http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/4087 2013-05-30T10:33:29Z 2013-05-30T00:00:00Z

RESIDENT MICROBIAL FLORA IN HUMAN ERYTHROCYTES An erythrocyte – like microorganism (ELM) was isolated in pure culture from 8 samples of blood taken from newborn umbilical cord, 50 samples of human blood for transfusion, and also in blood samples from 4 professors and 6 associate professors, who wanted to assure themselves in the discovery of new resident microbial flora, this time in human erythrocytes, about which there was no information in the medical literature. The size of ELM varied 1between 0.3 µm and 2.6 µm and because of this (with a size of 3.5 µm to 7.5 µm) 2 to 12 ELM cells could be seen (like in nest) in one erythrocyte after cultivation for 14 days at a temperature of 43°C in brain and heart liquid media. The electron microscope examination showed that ELM expulsed something like a cell nucleus being outside the human erythrocyte and it remained nucleus – free just like human red blood cells. This mimicry explained why ELM was able to multiply as a resident microbial flora in the human erythrocyte. ELM formed a unique red coating in the brain and heart media and very small grey colonies (which are almost invisible) on human blood agar after cultivation for 21 days at temperature 37°C, but not on sheep blood agar. Lysol as well as heat (70°C for 10 minutes) killed the ELM. The urease, lysine decarboxylase and catalase tests were positive. ELM produced acid from glucose and maltose, but not from sucrose and lactose. DNA could be demonstrated by the use of SDS polyacrilamide gel electrophoresis, but not by fluorescent stain because there was no nucleus in the ELM outside the erythrocytes. Sodium polyanetholsulphonate was bacteriostatic for ELM and due to this for the ELM isolation from blood the media should be prepared using sodium citrate.

2013-05-30T00:00:00Z http://dspace.mediu.edu.my:8181/xmlui/handle/123456789/4086 2013-05-30T10:32:59Z 2013-05-30T00:00:00Z

AN INCREASE OF THE TOTAL SOLIDIFICATION TEMPERATURE IN SALT MEDIA BY DEXTRAN PROTECTION An investigation was performed on the possibilities for an alteration of the total solidification temperature when preserving concentrated salt media (MBM, MEM, 199, HAM), used for the maintenance of animal viruses and cell cultures. It had been established that by the use of dextran 40000, this temperature could be increased with about 20°C. A protection in 3.6% concentration was recommended and the freezing to minus 40°C might be performed directly in the refrigerator for storage. The temperature should not be raised over minus 32°C during the stockage.

2013-05-30T00:00:00Z