| dc.creator |
Yruela Guerrero, Inmaculada |
|
| dc.creator |
Torrado Plou, Elena |
|
| dc.creator |
Roncel Gil, Mercedes |
|
| dc.creator |
Picorel Castaño, Rafael |
|
| dc.date |
2008-04-30T14:10:51Z |
|
| dc.date |
2008-04-30T14:10:51Z |
|
| dc.date |
2001-03 |
|
| dc.date.accessioned |
2017-01-31T01:06:38Z |
|
| dc.date.available |
2017-01-31T01:06:38Z |
|
| dc.identifier |
Photosynthesis Research 67(3): 199-206, 2001 |
|
| dc.identifier |
0166-8595 (print version) |
|
| dc.identifier |
1573-5079 (electronic version) |
|
| dc.identifier |
http://hdl.handle.net/10261/3956 |
|
| dc.identifier |
10.1023/A:1010682416016 |
|
| dc.identifier.uri |
http://dspace.mediu.edu.my:8181/xmlui/handle/10261/3956 |
|
| dc.description |
The definitive version is available at: http://www.springerlink.com/content/l304h18744r52202/fulltext.html |
|
| dc.description |
The light-induced difference absorption spectra associated to the photo-accumulation of reduced pheophytin a were studied in the isolated D1–D2–Cyt b559 complex in the presence of variable methyl viologen concentrations and different illumination conditions under anaerobiosis. Depending on the methyl viologen/reaction centre ratio, the relative intensities of the spectral bands at 681.5±0.5, 667.0±0.5 and 542.5±0.5 nm were modified. The reduced pheophytin a located at the D1-branch of the complex absorbs at 681.7±0.5 nm, and at least two additional pigment species contribute to the Qy band of the difference absorption spectra with maxima at 667.0±0.5 and 680.5±0.5 nm. We propose the additional species correspond to a peripheral chlorophyll a and the pheophytin a located at the D2-branch of the complex, respectively. The blue absorbing chlorophyll at 667 nm is susceptible to chemical redox changes with a midpoint reduction potential of +470 mV. The Qx absorption bands of both pheophytins localised at the D2- and D1-branch of the D1–D2–Cyt b559 complex were at 540.7±0.5 and 542.9±0.5, respectively. The results indicated that the two pheophytin molecules can be photoreduced in the D1–D2–Cyt b559 complex in certain experimental conditions. |
|
| dc.description |
This work was supported by the Dirección General de Investigación Científica y Técnica (Grant PB98-1632). |
|
| dc.description |
Peer reviewed |
|
| dc.format |
321506 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
eng |
|
| dc.publisher |
Springer |
|
| dc.rights |
openAccess |
|
| dc.subject |
Absorption |
|
| dc.subject |
Chlorophyll |
|
| dc.subject |
Pheophytin |
|
| dc.subject |
Photosystem II |
|
| dc.subject |
Reaction centre |
|
| dc.subject |
Spectroscopy |
|
| dc.title |
Light-induced absorption spectra of the D1-D2-cytochrome b559 complex of the Photosystem II: Effect of methyl viologen concentration |
|
| dc.type |
Artículo |
|