| dc.creator |
Yuk, Inn Huam Yvonne. |
|
| dc.creator |
Wang, Daniel I.C. |
|
| dc.date |
2003-12-08T15:24:37Z |
|
| dc.date |
2003-12-08T15:24:37Z |
|
| dc.date |
2003-01 |
|
| dc.date.accessioned |
2013-10-09T02:32:27Z |
|
| dc.date.available |
2013-10-09T02:32:27Z |
|
| dc.date.issued |
2013-10-09 |
|
| dc.identifier |
http://hdl.handle.net/1721.1/3784 |
|
| dc.identifier.uri |
http://koha.mediu.edu.my:8181/xmlui/handle/1721 |
|
| dc.description |
N-linked glycosylation often imparts important properties to protein therapeutics. An essential step in this intracellular process is the transfer of oligosaccharide from dolichol monophosphate (Dol-P) to a potential glycosylation site. Variability in the success rate of this reaction affects the extent of protein glycosylation. The critical role of Dol-P suggests that its availability may influence the extent of glycosylation by limiting the pool of lipid-linked oligosaccharides (LLOs), the glycosyl donor. To test this hypothesis, the impact of Dol-P supplementation on protein glycosylation in Chinese hamster ovary (CHO) cells was investigated. Although exogenous Dol-P was incorporated by CHO cells and processed into LLOs in a dose-dependent manner, Dol-P supplementation had no marked effects on LLO or overall cellular glycosylation levels. While concentrations of exogenous Dol-P exceeding 100 µg/ml were detrimental to CHO cell viability, maximum non-toxic supplemental doses of Dol-P had no significant impact on the glycosylation of recombinant interferon-γ produced by batch cultures of CHO cells. These results show that glycosylation in CHO cells cannot be readily enhanced by Dol-P feeding under normal culture conditions. |
|
| dc.description |
Singapore-MIT Alliance (SMA) |
|
| dc.format |
9846221 bytes |
|
| dc.format |
application/pdf |
|
| dc.language |
en_US |
|
| dc.relation |
Molecular Engineering of Biological and Chemical Systems (MEBCS); |
|
| dc.subject |
batch culture |
|
| dc.subject |
interferon-γ |
|
| dc.subject |
lipid-linked oligosaccharide |
|
| dc.title |
Glycosylation by Chinese Hamster Ovary Cells in Dolichol Phosphate-Supplemented Cultures |
|
| dc.type |
Article |
|