DSpace Repository

Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination

Show simple item record

dc.contributor DelCurto, Timothy
dc.contributor Mathews, Christopher
dc.contributor Stormshak, Fredrick
dc.contributor Menino, Alfred
dc.contributor Mueller, Chad
dc.contributor Sullivan, Dan
dc.date 2005-12-05T18:48:21Z
dc.date 2005-12-05T18:48:21Z
dc.date 2005-11-14
dc.date 2005-12-05T18:48:21Z
dc.date.accessioned 2013-10-16T07:28:57Z
dc.date.available 2013-10-16T07:28:57Z
dc.date.issued 2013-10-16
dc.identifier http://hdl.handle.net/1957/596
dc.identifier.uri http://koha.mediu.edu.my:8181/xmlui/handle/1957/596
dc.description Graduation date: 2006
dc.description Our objectives were to determine 1) the concentration of prostaglandin F2α (PGF2α) in whole and extended bovine semen, 2) if concentrations of prostaglandins in extended bovine semen are correlated to fertility, and 3) if PGF2α administered at the time of artificial insemination would improve conception rate. Concentration of PGF2α tended to be only slightly greater for whole compared to extended semen. To elucidate why PGF2α levels were comparable, semen was extended at eight dilution rates. Prostaglandin F2α in sub-samples, collected during extension, decreased at higher dilution rates and later steps of extension. To quantify PGF2α synthesized during extension, quantities of PGF2α in semen and the diluent were subtracted from each step. Higher dilution rates reduced the final amount of PGF2α synthesized. Initial PGF2α concentration was greater in whole semen compared to seminal plasma; however, when extended at three dilution rates quantity of PGF2α synthesized during extension was greater for semen compared to seminal plasma, indicating less disparity than for original samples. Concentrations of prostaglandin E2 (PGE2) and PGF2α in extended semen and the ratio of PGE2 to PGF2α were compared to a fertility rating. The ratio of PGE2 to PGF2α was not correlated to the fertility rating. Semen was extended to contain either 0, 500, or 5000 pg/ml of exogenous PGF2α. Exogenous PGF2α did not affect post-thaw motility or proportion of normal spermatozoa. In vitro fertilization did not differ between treatments. Analysis of cleavage rate per embryo revealed a bull x semen treatment interaction. First service conception rate of cows inseminated with the PGF2α-enhanced semen was affected by semen treatment and technician. Beef heifers inseminated with subfertile semen and dairy cows inseminated with normal fertility semen and treated with PGF2α after insemination displayed higher conception rates compared to control animals. These data suggest that, although extension reduces the concentration of many seminal components, PGF2α synthesis during extension results in concentrations similar to whole semen. Seminal prostaglandin concentrations may be related to fertilizing capability of the semen, and exogenous PGF2α administered at the time of insemination may improve conception rate.
dc.language en_US
dc.subject bovine
dc.subject semen
dc.subject prostaglandin F2alpha
dc.subject fertility
dc.subject artificial insemination
dc.subject conception rate
dc.title Quantities of prostaglandins in whole and extended bovine semen and their potential effect on fertility following insemination
dc.type Thesis

Files in this item

Files Size Format View

There are no files associated with this item.

This item appears in the following Collection(s)

Show simple item record

Search DSpace

Advanced Search


My Account